Vector:Plasmid DNA preparation: 13th april

Preparation of Plasmid DNA A plasmid preparation is a method used to extract and purify plasmid DNA. Many methods have been developed to purify plasmid DNA from bacteria. These methods basically involve three major steps Growth of the bacterial cell Harvesting and lysis of the bacteria Purification of plasmid DNA Growth of the bacterial cell [...]

Purification of total cell DNA: 7th april

Purification of DNA DNA is the basic constituent of any living being, and some viruses too. The isolation and purification of DNA is a significant step in any molecular biological technique. We are to discuss about the mentioned three. Total cell DNA preparation Preparation of plasmid DNA Preparation of bacteriophage DNA Preparation of Total Cell [...]

Vector: Bacteriophage lambda and M13: 7th april

Bacteriophage Definition They are one of the types of virus that attacks the bacteria and infects it. It was first reported by Frederick Twort, a british biologist and later by Felix d’Herelle, a French microbiologist. Diagram of phage Characteristic features of a bacteriophage They have a typical structure of outer protein capsid enclosing a genetic [...]

Plasmids: lecture notes on 17th march

Plasmids It is an extra chromosomal DNA molecule separate from the chromosomal DNA which is able to replicate independently of the chromosomal DNA. They vary from 1kb to 1000kb The term plasmid was first introduced by the American molecular biologist Joshua Lederberg in 1952 The plasmid doesnot contribute to the genome of the bacterial cell [...]

Vector part I: 17th march

Vector In molecular biology, a vector is a DNA molecule used as a vehicle to transfer foreign genetic material into another cell. The four major types of vectors are plasmids, bacteriophages and other viruses, cosmids, and artificial chromosomes. Common to all engineered vectors are an origin of replication, a multicloning site, and a selectable marker. [...]

Basics of gene cloning: 17th march

The basic steps in Gene Cloning Preparation of Pure sample of DNA from the organism of desire Cutting of the DNA molecule using Restriction enzymes to form fragments Analysis of DNA fragments using electrophoresis Identification of suitable vectors for insertion of the gene (DNA molecule). Joining of the two DNA molecules, I.e the fragmented DNA [...]

Genetic engineering introduction

What is Genetic Engineering? Genetic engineering, recombinant DNA technology, genetic modification or manipulation (GM) and gene splicing are terms that apply to the direct manipulation of an organism’s genes. The term “genetic engineering” was coined in Jack Williamson’s science fiction novel Dragon’s Island, published in 1951, two years before James Watson and Francis Crick showed [...]

Fiber optic cables on 3rd of july

Notes continued from transmission media….. Fiber Optic cables Fiber optics is defined as a technology that uses glass or plastic threads to transmit data. A fiber optic cable consists of a bundle of glass threads, each of which is capable of transmitting messages modulated onto light waves. It is completely base on the principle of [...]

Data communication- Transmission Media

Transmission Media Physical layer transmits a raw bit string from one machine to another. Various physical media used for the actual transmission are grouped into groups Guided media (copper wire, fiber optics) Unguided media (radio waves, laser etc) Guided Media Magnetic Media A common way to transfer data from one computer to another is to [...]