Basics of gene cloning: 17th march

The basic steps in Gene Cloning

1. Preparation of Pure sample of DNA from the organism of desire
2. Cutting of the DNA molecule using Restriction enzymes to form fragments
3. Analysis of DNA fragments using electrophoresis
4. Identification of suitable vectors for insertion of the gene (DNA molecule).
5. Joining of the two DNA molecules, I.e the fragmented DNA here and the plasmid.
6. Introduction of the DNA molecule, i.e the recombinant vector or the chimera, into the host cell.
7. Identification of the cell containing the recombinant DNA, through screening techniques

An animation of Gene cloning…

For further notes,

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